| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Miller, Devony | FR | Bluffton | 5:42.00 | |
| 2 | Matteson, Jennie | FR | Bluffton | 5:42.00 | |
| 3 | Volkman, Aleea | FR | Lake Erie | 5:45.00 | |
| 4 | Ashley, Erin | FR | Cedarville | 5:49.00 | |
| 5 | Aurand, Taylor | FR | Bluffton | 6:00.00 | |
| 6 | Suffel, Kiera | FR | Bluffton | 6:25.00 | |
| 7 | Fetters, Stephanie | SO | Wilmington (Ohio) | NT |