| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Wilmington (Ohio) |
A | Covington | Henson | Klosterman | Gilmore | 3:21.77 |
| 2 | Walsh |
A | Harder | Contrucci | Richmond | Ostheimer | 3:25.34 |
| 3 | Findlay |
A | Kolb | Lemaster | Sesay | Reason | 3:15.45 |
| 4 | Ashland |
A | Rager | Jones | Wood | Phillips | 3:06.12 |
| 5 | Mount Union |
A | McBride | Gill | Campbell | Knoch | 3:08.10 |
| 6 | Otterbein |
A | Booker | Engram | Hatfield | Lynch | 3:18.33 |
| 7 | Lake Erie |
A | Jackson | Souverain | Harvey | Johnson | DNS |
| 8 | Ashland |
B | Roberts | Vaughan | Pinkerton | Schuster | 3:24.00 |